RHOA Protein (Myc-DYKDDDDK Tag)
Quick Overview for RHOA Protein (Myc-DYKDDDDK Tag) (ABIN2730844)
Target
See all RHOA ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This RHOA protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RhoA protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ras Homolog Gene Family, Member A (RHOA) (AA 61-193) protein (His-IF2DI Tag)
RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) protein (His tag)RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) protein (GST tag)RHOA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Ras Homolog Gene Family, Member A (RHOA) (AA 1-191), (partial) protein (GST tag)RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % WB, SDS, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RHOA (Ras Homolog Gene Family, Member A (RHOA))
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Alternative Name
- Rhoa
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Background
- This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
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Molecular Weight
- 21.6 kDa
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NCBI Accession
- NP_001655
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Pathways
- Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome
Target
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