Riboflavin Kinase Protein (RFK) (Myc-DYKDDDDK Tag)
Quick Overview for Riboflavin Kinase Protein (RFK) (Myc-DYKDDDDK Tag) (ABIN2730888)
Target
See all Riboflavin Kinase (RFK) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Riboflavin Kinase protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Riboflavin kinase (RFK) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Riboflavin Kinase (RFK) (AA 1-155) protein (His tag)
custom-made RFK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Riboflavin Kinase (RFK) (AA 1-155) protein (His tag)custom-made RFK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Riboflavin Kinase (RFK) (AA 1-162) protein (GST tag)RFK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Riboflavin Kinase (RFK) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by reducing SDS-PAGE.
Riboflavin Kinase (RFK) (AA 1-162) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Riboflavin Kinase (RFK)
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Background
- Riboflavin kinase (RFK EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009].
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Molecular Weight
- 17.4 kDa
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NCBI Accession
- NP_060809
Target
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