SERPINB2 Protein (Transcript Variant 2)
Quick Overview for SERPINB2 Protein (Transcript Variant 2) (ABIN2731773)
Target
See all SERPINB2 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human SERPINB2 / PAI2 (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37C. Half maximal inhibition against 1.0 ug/mL of single chain tPA was obtained at a concentration of 1.0 ug/mL
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Plasminogen Activator Inhibitor 2 (SERPINB2) (AA 1-415) protein (His tag)
custom-made SERPINB2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Plasminogen Activator Inhibitor 2 (SERPINB2) (AA 1-415) protein (His tag)custom-made SERPINB2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Plasminogen Activator Inhibitor 2 (SERPINB2) (AA 1-415) protein (GST tag)SERPINB2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- SERPINB2 (Plasminogen Activator Inhibitor 2 (SERPINB2))
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Alternative Name
- Serpinb2,pai2
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Target Type
- Amino Acid
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Background
- Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 45.6 kDa
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NCBI Accession
- NP_002566
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Pathways
- Autophagy
Target
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