Skeletal Troponin T protein (DYKDDDDK Tag)
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- Target
- Skeletal Troponin T
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- DYKDDDDK Tag
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Skeletal muscle Troponin T (full length, C-term DDK tag, transcript variant 1) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Skeletal Troponin T
- Alternative Name
- Skeletal Muscle Troponin T
- Background
- Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 32.8 kDa
- NCBI Accession
- NP_003274
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