Skeletal Troponin T protein (DYKDDDDK Tag)
This Recombinant protein is produced in Insect cells (Sf9).
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Catalog No. ABIN2732112
Quick Overview for Skeletal Troponin T protein (DYKDDDDK Tag) (ABIN2732112)
Target
Skeletal Troponin T
Protein Type
Recombinant
Origin
Human
Source
Insect cells (Sf9)
Application
Antibody Production (AbP), Standard (STD)
Purity
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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Purification tag / Conjugate
- DYKDDDDK Tag
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Characteristics
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- Recombinant human Skeletal muscle Troponin T (full length, C-term DDK tag, transcript variant 1) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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Skeletal Troponin T (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Skeletal Troponin T
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Alternative Name
- Skeletal Muscle Troponin T
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Background
- Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 32.8 kDa
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NCBI Accession
- NP_003274
Target
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