SORD Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732375

Recombinant SORD protein expressed in HEK-293 Cells.

Quick Overview for SORD Protein (Myc-DYKDDDDK Tag) (ABIN2732375)

Target: SORD (Sorbitol Dehydrogenase (SORD))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This SORD protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human SORD protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SORD (Sorbitol Dehydrogenase (SORD))

Alternative Name: Sord

Background: Sorbitol dehydrogenase (SORD EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1 MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010].

Molecular Weight: 38.1 kDa

NCBI Accession: NP_003095