Sp3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732480

Recombinant Sp3 protein expressed in HEK-293 Cells.

Quick Overview for Sp3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2732480)

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This Sp3 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human SP3 / SPR-2 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Alternative Name: Sp3,spr-2

Background: This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

Molecular Weight: 81.7 kDa

NCBI Accession: NP_003102