T Brachyury Protein protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2733143

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for T Brachyury Protein protein (Myc-DYKDDDDK Tag) (ABIN2733143)

Target: T Brachyury Protein

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: Myc-DYKDDDDK Tag

Characteristics:

• Recombinant human T (Brachyury protein) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: T Brachyury Protein

Alternative Name: T (Brachyury Protein)

Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 47.3 kDa

NCBI Accession: NP_003172

Pathways: Tube Formation