TAF12 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for TAF12 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2733193)
Target
See all TAF12 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This TAF12 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human TAF12 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (AA 1-161) protein (His tag)
custom-made TAF12 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (AA 1-161) protein (His tag)custom-made TAF12 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (AA 1-161) protein (GST tag)TAF12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (AA 1-161), (full length) protein (His-SUMO Tag)TAF12 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- TAF12 (TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12))
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Alternative Name
- Taf12
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Background
- Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.
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Molecular Weight
- 17.7 kDa
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NCBI Accession
- NP_005635
Target
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