LTA Protein (Transcript Variant 2)

Catalog No. ABIN2733938

Recombinant LTA protein expressed in Escherichia coli (E. coli).

Quick Overview for LTA Protein (Transcript Variant 2) (ABIN2733938)

Target: LTA (Lymphotoxin-alpha (LTA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Chemical

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human TNF-beta / Lymphotoxin-alpha (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: Tnf-Beta,lymphotoxin-alpha

Target Type: Chemical

Background: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 18.6 kDa

NCBI Accession: NP_000586

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process