NFE4 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734129

This Recombinant NFE4 protein is expressed in HEK-293 Cells.

Quick Overview for NFE4 Protein (Myc-DYKDDDDK Tag) (ABIN2734129)

Target: NFE4 (Transcription Factor NF-E4 (NFE4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This NFE4 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Transcription factor NF-E4 (NF-E4) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NFE4 (Transcription Factor NF-E4 (NFE4))

Alternative Name: Transcription Factor Nf-e4 (Nf-e4)

Background: The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand.

Molecular Weight: 18.9 kDa

NCBI Accession: NP_001078855