Tropomyosin Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for Tropomyosin Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2734387)
Target
See all Tropomyosin (TPM1) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This Tropomyosin protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Tropomyosin-1 (TPM1) (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Tropomyosin 1 (Alpha) (TPM1) (AA 1-284) protein (His tag)
custom-made TPM1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tropomyosin 1 (Alpha) (TPM1) (AA 1-284) protein (His tag)custom-made TPM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tropomyosin 1 (Alpha) (TPM1) (AA 1-245) protein (GST tag)TPM1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Tropomyosin 1 (Alpha) (TPM1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)TPM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Tropomyosin 1 (Alpha) (TPM1) (Transcript Variant 5) protein (Myc-DYKDDDDK Tag)TPM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))
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Alternative Name
- Tropomyosin-1 (Tpm1)
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Background
- This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
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Molecular Weight
- 32.5 kDa
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NCBI Accession
- NP_001018007
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Pathways
- Regulation of Actin Filament Polymerization
Target
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