TWIST1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734647

This Recombinant TWIST1 protein is produced in HEK-293 Cells.

Quick Overview for TWIST1 Protein (Myc-DYKDDDDK Tag) (ABIN2734647)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This TWIST1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human TWIST1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: Twist1

Background: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

Molecular Weight: 20.8 kDa

NCBI Accession: NP_000465

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity