UBE2I Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for UBE2I Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2734775)
Target
See all UBE2I ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This UBE2I protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human UBC9 / UBE2I (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-158) protein (GST tag)
UBE2I Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-157), (partial) protein (GST tag)UBE2I Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)UBE2I Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)UBE2I Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2I Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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Alternative Name
- Ubc9,ube2i
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Background
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
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Molecular Weight
- 17.8 kDa
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NCBI Accession
- NP_003336
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Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
Target
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