UBE2I Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734776

This Recombinant UBE2I protein is produced in HEK-293 Cells.

Quick Overview for UBE2I Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2734776)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This UBE2I protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human UBC9 / UBE2I (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: Ubc9,ube2i

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 17.8 kDa

NCBI Accession: NP_919235

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway