UBE2N Protein (Myc-DYKDDDDK Tag)
Quick Overview for UBE2N Protein (Myc-DYKDDDDK Tag) (ABIN2734860)
Target
See all UBE2N ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This UBE2N protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human UBE2N / BLU protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (His tag)
custom-made UBE2N Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 2-152) protein (His tag)UBE2N Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (GST tag)UBE2N Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (GST tag)UBE2N Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (His tag)UBE2N Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))
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Alternative Name
- Ube2n,blu
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Background
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
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Molecular Weight
- 17 kDa
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NCBI Accession
- NP_003339
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway
Target
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