UBE2N Protein (AA 1-152) (His tag)
Quick Overview for UBE2N Protein (AA 1-152) (His tag) (ABIN666632)
Target
See all UBE2N ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-152
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Purification tag / Conjugate
- This UBE2N protein is labelled with His tag.
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Sequence
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MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI -
Characteristics
- UBE2N, 1-152aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (His tag)
custom-made UBE2N Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (GST tag)UBE2N Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 2-152) protein (His-IF2DI Tag)UBE2N Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (GST tag)UBE2N Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2N (UBE2N) protein (Myc-DYKDDDDK Tag)UBE2N Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))
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Alternative Name
- Ubc13/UBE2N
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Background
- UBE2N, also known as UBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression, cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage. Recombinant human UBE2N protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 19.3 kDa (172aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_003339
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway
Target
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