UPF3B Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735037

This Recombinant UPF3B protein is produced in HEK-293 Cells.

Quick Overview for UPF3B Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2735037)

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This UPF3B protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human UPF3B / RENT3B (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Alternative Name: Upf3b,rent3b

Background: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Molecular Weight: 56 kDa

NCBI Accession: NP_075386