VASP Protein (Myc-DYKDDDDK Tag)
Quick Overview for VASP Protein (Myc-DYKDDDDK Tag) (ABIN2735193)
Target
See all VASP ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This VASP protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human VASP protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- VASP (Vasodilator-Stimulated phosphoprotein (VASP))
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Alternative Name
- Vasp
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Background
- Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
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Molecular Weight
- 39.6 kDa
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NCBI Accession
- NP_003361
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Pathways
- TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
Target
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