Vinculin Protein (VCL) (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735299

This Recombinant Vinculin protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for Vinculin Protein (VCL) (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2735299)

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This Vinculin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Vinculin (VCL) (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Beppu, Sawai, Satoh, Mori, Kazami, Misawa, Shibuya, Ishibashi, Sogawa, Kado, Kodera, Nomura, Kuwabara: "Autoantibodies against vinculin in patients with chronic inflammatory demyelinating polyneuropathy." in: Journal of neuroimmunology, Vol. 287, pp. 9-15, (2015) (PubMed).

Target Details

Target: Vinculin (VCL)

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Molecular Weight: 116.5 kDa

NCBI Accession: NP_003364

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2