Vinculin Protein (VCL) (AA 777-1066) (GST tag)

Catalog No. ABIN7125717

This Recombinant Vinculin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Vinculin Protein (VCL) (AA 777-1066) (GST tag) (ABIN7125717)

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 777-1066

Purification tag / Conjugate: This Vinculin protein is labelled with GST tag.

Purpose: Recombinant Human VCL

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Vinculin (VCL)

Alternative Name: VCL

Background: CMD1W, Metavinculin, MVCL, VCL, Vinculin

Molecular Weight: 60.5 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2