WIT1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735592

This Recombinant WIT1 protein is produced in HEK-293 Cells.

Quick Overview for WIT1 Protein (Myc-DYKDDDDK Tag) (ABIN2735592)

Target: WIT1 (WT1-AS) (WT1 Antisense RNA (WT1-AS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This WIT1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Wilms tumor upstream neighbor 1 (WIT1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: WIT1 (WT1-AS) (WT1 Antisense RNA (WT1-AS))

Alternative Name: Wilms Tumor Upstream Neighbor 1 (Wit1)

Background: This gene is located upstream of the Wilms tumor 1 (WT1) gene these two genes are bi-directionally transcribed from the same promoter region. This gene is imprinted in kidney, with preferential expression from the paternal allele. Imprinting defects at chromosome 11p13 may contribute to tumorigenesis.

Molecular Weight: 9.9 kDa

NCBI Accession: NP_056939