ZYX Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for ZYX Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2736196)
Target
See all ZYX ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This ZYX protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Zyxin (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Zyxin (ZYX) (AA 2-168) protein (His-IF2DI Tag)
ZYX Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Zyxin (ZYX) (AA 1-572) protein (GST tag)ZYX Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Zyxin (ZYX) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ZYX Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ZYX (Zyxin (ZYX))
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Alternative Name
- Zyxin
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Background
- Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
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Molecular Weight
- 61.1 kDa
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NCBI Accession
- NP_003452
Target
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