BAFF Protein (AA 136-285) (Fc Tag)

Catalog No. ABIN2745615

This Recombinant BAFF protein is produced in CHO Cells.

Quick Overview for BAFF Protein (AA 136-285) (Fc Tag) (ABIN2745615)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: CHO Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 136-285

Purification tag / Conjugate: This BAFF protein is labelled with Fc Tag.

Purpose: Fc (human):BAFF (human) (rec.)

Specificity: Binds to human and mouse BAFF-R, TACI and BCMA.

Cross-Reactivity: Human, Mouse

Characteristics: The receptor-binding domain of human BAFF (aa 136-285) is fused at the N-terminus to the Fc portion of human IgG1.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Rescues the production of mature follicular and marginal zone B cells. - in vitro: Addition of 1-100 ng/ml of Fc-BAFF induces B cell survival in a dose-dependent manner. - in vivo: Fc-BAFF treatment rescues mature B cell development in BAFF deficient mice. Mice are injected at day 0 with 100 μg Fc-BAFF i.v. and at day 14 with 50 μg Fc-BAFF i.p.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: Lot specific

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: :BAFF

Background:

BLyS, TALL-1, CD257, B Cell Activating Factor, TNFSF13B, Tumor Necrosis Factor Ligand Superfamily Member 13B

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers.

Molecular Weight: ~50kDa (SDS-PAGE, reducing conditions)~100kDa (SDS-PAGE, non-reducing conditions)~300kDa (SDS-PAGE, native conditions, multimerizes and forms hexamers)

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response