CLEC7A Protein (AA 72-244) (Fc Tag)

Catalog No. ABIN2745633

Product Details

Target: CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))

Protein Type: Recombinant

Protein Characteristics: AA 72-244

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CLEC7A protein is labelled with Fc Tag.

Application: SDS-PAGE (SDS)

Purpose: Fc (human):Dectin-1 (mouse) (rec.)

Cross-Reactivity: Mouse

Characteristics: Mouse dectin-1 (aa 72-244) is fused at the C-terminus to the Fc portion of human IgG1.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile water.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))

Alternative Name: :Dectin-1 (CLEC7A Products)

Background:

C-type Lectin Domain Family 7 Member A, Beta-Glucan Receptor, Dendritic Cell-associated C-type Lectin 1, C-type Lectin Superfamily Member 12, DC-associated C-type Lectin 1

Dectin-1 and dectin-2 are type II transmembrane proteins of the C-type lectin family. They contain single carbohydrate recognition domains (CRDs) in their extracellular region. Dectin-1 and dectin-2 are expressed in macrophages and dendritic cells. Dectin-1 recognizes beta-glucans with its CRD and signals through its immunoreceptor tyrosine-based activation motif (ITAM)-like motif in the cytoplasmic domain, whereas dectin-2 recognizes alpha-mannans and transduces its signal through association with the ITAM-containing Fc receptor gamma-chain. Dectin-1 controls fungal infection by sensing beta-glucans and by mounting a Th17-mediated immune responses. Dectin-1 seems to detect also non-fungal ligands such as invertebrate tropomyosin. Dectin-1 by binding to non-fungal ligands controls type 2 immunity in the lungs in response to the dust mite aeroallergen through the regulation of IL-33-driven IL-13 production from innate lymphoid cells.

Molecular Weight: ~56kDa (SDS-PAGE)

UniProt: Q6QLQ4

Pathways: Activation of Innate immune Response