NAPRT1 Protein (His tag)
Quick Overview for NAPRT1 Protein (His tag) (ABIN2745752)
Target
See all NAPRT1 (NAPRT) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This NAPRT1 protein is labelled with His tag.
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Purpose
- NAPRTase (human) (rec.) (His)
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Cross-Reactivity
- Human
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Characteristics
- Human NAPRTase is fused at the N-terminus to a His-tag.
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Biological Activity Comment
- 0.043μmol/min/mg: hNAPRTase activity was measured by a custom coupled enzymatic assay which uses a secondary enzyme for the quantification of the PRPP consumed by hNAPRTase. The reaction buffer contained 50 mM Hepes pH 7.5, 500 μM PRPP, 100 μM nicotinic acid, 20 mM MgCl2, 2.5 mM DTT and hNAPRTase at 5 μM, 25°C, spectrophotometrical monitoring.
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Nicotinate phosphoribosyltransferase (NAPRT) (AA 1-538) protein (His tag)
custom-made NAPRT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nicotinate phosphoribosyltransferase (NAPRT) (AA 1-538) protein (His tag)custom-made NAPRT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nicotinate phosphoribosyltransferase (NAPRT) (AA 1-466) protein (GST tag)NAPRT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nicotinate phosphoribosyltransferase (NAPRT) protein (His tag)NAPRT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- In 50 mM HEPES/KOH pH 7.5 containing 300 mM potassium chloride and 10 mM DTT.
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Handling Advice
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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Expiry Date
- 6 months
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- NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))
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Alternative Name
- NAPRTase
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Background
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Nicotinate Phosphoribosyltransferase, FHA-HIT-interacting Protein, FHIP, EC 2.4.2.11
Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
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Molecular Weight
- ~58.0kDa
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UniProt
- Q6XQN6
Target
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