NAPRT1 Protein

Catalog No. ABIN2745753

Product Details

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Protein Type: Recombinant

Origin: Bacillus subtilis

Source: Synthetic

Purpose: NAPRTase (B. subtilis) (rec.)

Characteristics: Full-length NAPRTase (Bacillus subtilis).

Purity: >98 % (SDS-PAGE)

Biological Activity Comment: ~0.8 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinic acid mononucleotide (NaMN) per min.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Lot specific

Concentration: Lot specific

Buffer: in 20 mM TRIS-HCl, pH 7.8 containing 50 mM sodium chloride.

Handling Advice: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))

Alternative Name: NAPRTase (NAPRT Products)

Background:

Nicotinate Phosphoribosyltransferase, pncB, yueK, EC 2.4.2.11

Nicotinate Phosphoribosyltransferase (NAPRTase) is involved in the biological processes of pyridine nucleotide biosynthesis and nicotinate nucleotide salvage and functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.

Molecular Weight: ~56.0kDa

UniProt: O32090