PARG Protein (PARG) (His tag)
Quick Overview for PARG Protein (PARG) (His tag) (ABIN2745792)
Target
See all PARG ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PARG protein is labelled with His tag.
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Purpose
- PARG (human) (rec.) (His) (highly active)
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Cross-Reactivity
- Human
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Characteristics
- Human PARG is fused to a His-tag.
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Biological Activity Comment
- ~100ng is required for poly(ADP-ribose) degradation assays.
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PARG (PARG) (AA 1-969) protein (His tag)
custom-made PARG Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PARG (PARG) (AA 1-976) protein (His tag)custom-made PARG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PARG (PARG) (AA 357-467) protein (GST tag)PARG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
PARG (PARG) (AA 1-976) protein (GST tag)PARG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50 mM imidazole and 10 % glycerol.
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Handling Advice
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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Expiry Date
- 6 months
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- PARG
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Alternative Name
- PARG
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Background
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Poly(ADP-ribose) Glycohydrolase
Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
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UniProt
- Q86W56
Target
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