VEGF 165 (Active) Protein
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- Target
- VEGF 165
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Purpose
- VEGF 165 (human) (rec.)
- Cross-Reactivity
- Human
- Characteristics
- Human VEGF 165 (165aa).
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.1ng/μg protein.
- Biological Activity Comment
- The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.
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VEGF 165 protein
Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
VEGF 165 (AA 1-191) protein (His tag)Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
VEGF 165 proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 50 mM acetic acid to a concentration not lower than 50 μg/mL.
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- VEGF 165
- Background
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Vascular Endothelial Growth Factor, VEGF-A, VPF
Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.
- Molecular Weight
- ~38.2kDa
- UniProt
- P15692-4
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