VEGF 165 (AA 207-371) protein (His tag)

Catalog No. ABIN666798

Product Details

Target: VEGF 165

Protein Type: Recombinant

Protein Characteristics: AA 207-371

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Characteristics: VEGF165, 207-371aa, Human, His tag, E.coli

Purity: > 90 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl(pH8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA

Storage: 4 °C

Target Details

Target: VEGF 165

Background: Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Synonyms: Vascular endothelial growth factor A isoform d, VPF, VEGF, VEGF-A. NCBI no.: NP_001020539

Molecular Weight: 21.3kDa (185aa), conformed by MALDI-TOF