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VEGF 165 Protein

Name: VEGF 165 Protein
Brand: antibodies-online
SKU: ABIN7275824
Price: 345.38 USD
Availability: InStock

Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Human VEGF165 on Tris-Bis PAGE under reduced condition. (VEGF 165 Protein)

SDS

Immobilized Human VEGF165, No Tag at 5 μg/mL (100 μL/well) on the plate. (VEGF 165 Protein)

ELISA

SEC-HPLC

Immobilized Human VEGF165 at 1 μg/mL (100 μL/well) on the plate. (VEGF 165 Protein)

ELISA

Human VEGF165 stimulates proliferation of human umbilical vein endothelial cells (HUVEC) with the ED50 for this effect is 2. (VEGF 165 Protein)

ProA

ELISA

6 images

Catalog No. ABIN7275824

Datasheet as PDF

Target

VEGF 165

Protein Type

Recombinant
Origin
16

3

2
Human
Source
10

6

1

1

1

1

1
HEK-293 Cells

Sequence

Ala27-Arg191

Purity

> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility

0.22 μm filtered

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity Comment

Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail.
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)
Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active

VEGF 165 (AA 27-191) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (AA 207-371) protein (His tag)
Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS

VEGF 165 protein
Origin: Rat Host: Escherichia coli (E. coli) Recombinant

VEGF 165 protein
Origin: Mouse Host: Escherichia coli (E. coli) Recombinant

VEGF 165 (Active) protein
Origin: Mouse Host: Escherichia coli (E. coli) > 95 % by SDS-PAGE and HPLC analyses. Active

VEGF 165 (Active) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % (SDS-PAGE) SDS Active

VEGF 165 (AA 27-190) (Active) protein
Origin: Rat Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

VEGF 165 (Active) protein
Origin: Human Host: Insect cells (Sf9) Recombinant >95 % (SDS-PAGE) SDS Active

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage

4 °C,-80 °C

Storage Comment

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date

12 months
Target

VEGF 165

Alternative Name

VEGF165

Background

VEGF, VEGFA, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609,Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

Molecular Weight

19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.