VEGF 165 (Active) Protein

Catalog No. ABIN988289

Product Details

Target: VEGF 165

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPR

Characteristics: Measured by its ability to stimulate 3H-thymidine incorporation in HUVE cells. The ED50 for this effect is typically 2 - 4 ng/mL,corresponding to a specific activity of > 2.5×105 units/mg.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rmVEGF165 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: VEGF 165

Alternative Name: Vascular Endothelial Growth Factor 165 (VEGF165)

Background: VEGF was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent angiogenesis inducer. Synonym: Vascular Endothelial Growth Factor 165 (VEGF165), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.

Molecular Weight: Recombinant murine VEGF165 is a 38.8 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.