Proteoglycan 3 Protein (PRG3) (AA 18-225) (His tag)

Catalog No. ABIN2746048

Recombinant Proteoglycan 3 protein expressed in Human Cells.

Quick Overview for Proteoglycan 3 Protein (PRG3) (AA 18-225) (His tag) (ABIN2746048)

Target: Proteoglycan 3 (PRG3)

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 18-225

Purification tag / Conjugate: This Proteoglycan 3 protein is labelled with His tag.

Purpose: Recombinant Human Proteoglycan 3/PRG3 (C-6His)

Sequence: LHLENDAPHL ESLETQADLG QDLDSSKEQE RDLALTEEVI QAEGEEVKAS ACQDNFEDEE AMESDPAALD KDFQCPREED IVEVQGSPRC KTCRYLLVRT PKTFAEAQNV CSRCYGGNLV SIHDFNFNYR IQCCTSTVNQ AQVWIGGNLR GWFLWKRFCW TDGSHWNFAY WSPGQPGNGQ GSCVALCTKG GYWRRAQCDK QLPFVCSFVD HHHHHH

Characteristics: Recombinant Human Proteoglycan 3/PRG3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu18-Phe225) of Human PRG3 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Proteoglycan 3 (PRG3)

Alternative Name: Proteoglycan-3

Sub Type: Fusionprotein

Background: Proteoglycan 3, also known as Eosinophil major basic protein homolog, Prepro-major basic protein homolog, PRG3 and MBPH, contains one C-type lectin domain. Proteoglycans are a major component of the animal extracellular matrix. PRG3 localizes to the eosinophil secondary granule and is expressed in bone marrow, not detected in placenta. PRG3 has similar cytotoxic and cytostimulatory activities to PRG2/MBP. In vitro, PRG3 can stimulate neutrophil superoxide production and IL8 release, histamine and leukotriene C4 release from basophils.
Alternative Names: Proteoglycan 3, Eosinophil Major Basic Protein Homolog, Prepro-Major Basic Protein Homolog, Prepro-MBPH, PRG3, MBPH

Molecular Weight: 24.6 kDa

UniProt: Q9Y2Y8