UBE2B Protein (AA 1-152) (His tag)
Quick Overview for UBE2B Protein (AA 1-152) (His tag) (ABIN2746055)
Target
See all UBE2B ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-152
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Purification tag / Conjugate
- This UBE2B protein is labelled with His tag.
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Purpose
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B/HR6B (C-6His)
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Sequence
- MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DSLDHHHHHH
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Characteristics
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Ser152) of Human UBE2B fused with a polyhistidine tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 1-152) protein (His tag)
custom-made UBE2B Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 1-152) protein (His-IF2DI Tag)UBE2B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 1-152) protein (GST tag)UBE2B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2B (UBE2B) protein (Myc-DYKDDDDK Tag)UBE2B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))
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Alternative Name
- HR6B
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Sub Type
- Fusionprotein
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Target Type
- Viral Protein
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Background
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Ubiquitin-Conjugating Enzyme E2 B (UBE2B) is a member of the ubiquitin-conjugating enzyme family. UBE2B accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It is shown that UBE2B interacts with RAD18, UBR2, and WAC. UBE2B is required for post-replicative DNA damage repair. Additional, UBE2B plays a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 B, RAD6 Homolog B, HR6B, hHR6B, Ubiquitin Carrier Protein B, Ubiquitin-Conjugating Enzyme E2-17 kDa, Ubiquitin-Protein Ligase B, UBE2B, RAD6B -
Molecular Weight
- 18.3 kDa
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UniProt
- P63146
Target
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