MCAM Protein (AA 24-646) (His-DYKDDDDK-Strep II Tag)
Quick Overview for MCAM Protein (AA 24-646) (His-DYKDDDDK-Strep II Tag) (ABIN2752854)
Target
See all MCAM ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 24-646
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Purification tag / Conjugate
- This MCAM protein is labelled with His-DYKDDDDK-Strep II Tag.
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Purpose
- MCAM (Human) Recombinant Protein
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Sequence
- VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGVVIV AVIVCILVLA VLGAVLYFLY KKGKLPCRRS GKQEITLPPS RKSELVVEVK SDKLPEEMGL LQGSSGDKRA PGDQGEKYID LRH
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Characteristics
- Purified MCAM (NP_006491.2, 24 a.a. - 646 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
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Purification
- Transfection of pSuper-MCAM plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
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Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)
MCAM Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-563) protein (His tag)MCAM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) protein (GST tag)MCAM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)MCAM Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Transfection of pSuper-MCAM plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
Product Quality tested by: SDS-PAGE and Western Blot -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 10 μg/mL
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Buffer
- 100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
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Preservative
- Other preservative
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Alternative Name
- MCAM
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Background
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Full Gene Name: melanoma cell adhesion molecule
Synonyms: CD146,MUC18 -
Gene ID
- 4162
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NCBI Accession
- NM_006500
Target
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