Lipocalin 2 Protein (LCN2)

Catalog No. ABIN2830468

This Recombinant Lipocalin 2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Lipocalin 2 Protein (LCN2) (ABIN2830468)

Target: Lipocalin 2 (LCN2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: >95 % as determined by SDS-PAGE and HPLC.

Product Details

Sequence: QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG

Characteristics: Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/mL, corresponding to a specific activity of ＞ 2.0 x 106 IU/mg.

Sterility: 0.2 μm filtered

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM PB, pH 7.4, 150 mM NaCl

Storage: -20 °C

Target Details

Target: Lipocalin 2 (LCN2)

Alternative Name: LCN2

Background: Relevance: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. {ECO:0000269|PubMed:12453413}.
Synonyms: NGAL, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25

Molecular Weight: 41.0 kDa

UniProt: P80188

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis