TTLL2 Protein (AA 1-592) (Strep Tag)

Catalog No. ABIN3074948

Recombinant TTLL2 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for TTLL2 Protein (AA 1-592) (Strep Tag) (ABIN3074948)

Target: TTLL2 (Tubulin Tyrosine Ligase-Like Family, Member 2 (TTLL2))

Protein Type: Recombinant

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-592

Purification tag / Conjugate: This TTLL2 protein is labelled with Strep Tag.

Sequence: MRGRDLCSST QSQALGSLRT TTPAFTLNIP SEANHTEQPP AGLGARLQEA GVSIPPRRGR PTPTLEKKKK PHLMAEDEPS GALLKPLVFR VDETTPAVVQ SVLLERGWNK FDKQEQNAED WNLYWRTSSF RMTEHNSVKP WQQLNHHPGT TKLTRKDCLA KHLKHMRRMY GTSLYQFIPL TFVMPNDYTK FVAEYFQERQ MLGTKHSYWI CKPAELSRGR GILIFSDFKD FIFDDMYIVQ KYISNPLLIG RYKCDLRIYV CVTGFKPLTI YVYQEGLVRF ATEKFDLSNL QNNYAHLTNS SINKSGASYE KIKEVIGHGC KWTLSRFFSY LRSWDVDDLL LWKKIHRMVI LTILAIAPSV PFAANCFELF GFDILIDDNL KPWLLEVNYS PALTLDCSTD VLVKRKLVHD IIDLIYLNGL RNEGREASNA THGNSNIDAA KSDRGGLDAP DCLPYDSLSF TSRMYNEDDS VVEKAVSVRP EAAPASQLEG EMSGQDFHLS TREMPQSKPK LRSRHTPHKT LMPYASLFQS HSCKTKTSPC VLSDRGKAPD PQAGNFVLVF PFNEATLGAS RNGLNVKRII QELQKLMNKQ HS
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TTLL2 (Tubulin Tyrosine Ligase-Like Family, Member 2 (TTLL2))

Alternative Name: TTLL2

Background: Probable tubulin polyglutamylase TTLL2 (EC 6.-.-.-) (Testis-specific protein NYD-TSPG) (Tubulin--tyrosine ligase-like protein 2),FUNCTION: Probable tubulin polyglutamylase that generates side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of target proteins (By similarity). Similar to TTLL1, may acquire enzymatic activity only in complex with other proteins as it is most likely lacking domains important for autonomous activity (By similarity). Probably involved in the side-chain initiation step of the polyglutamylation reaction rather than the elongation step (By similarity). {ECO:0000250|UniProtKB:A4Q9E4, ECO:0000250|UniProtKB:Q91V51}.

Molecular Weight: 67.3 kDa

UniProt: Q9BWV7