ARHGEF10 Protein (AA 1-1369) (Strep Tag)

Catalog No. ABIN3089111

This Recombinant ARHGEF10 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for ARHGEF10 Protein (AA 1-1369) (Strep Tag) (ABIN3089111)

Target: ARHGEF10 (rho Guanine Nucleotide Exchange Factor (GEF) 10 (ARHGEF10))

Protein Type: Recombinant

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1369

Purification tag / Conjugate: This ARHGEF10 protein is labelled with Strep Tag.

Sequence: MRPPGFLSRA PSLNRAERGI WSCSMDQREP LPPAPAENEM KYDTNNNEEE EGEQFDFDSG DEIPEADRQA PSAPETGGAG ASEAPAPTGG EDGAGAETTP VAEPTKLVLP MKVNPYSVID ITPFQEDQPP TPVPSAEEEN VGLHVPCGYL VPVPCGYAVP SNLPLLLPAY SSPVIICATS LDEEAETPEV TEDRQPNSLS SEEPPTSEDQ VGREDSALAR WAADPANTAW MENPEEAIYD DVPRENSDSE PDEMIYDDVE NGDEGGNSSL EYGWSSSEFE SYEEQSDSEC KNGIPRSFLR SNHKKQLSHD LTRLKEHYEK KMRDLMASTV GVVEIQQLRQ KHELKMQKLV KAAKDGTKDG LERTRAAVKR GRSFIRTKSL IAQDHRSSLE EEQNLFIDVD CKHPEAILTP MPEGLSQQQV VRRYILGSVV DSEKNYVDAL KRILEQYEKP LSEMEPKVLS ERKLKTVFYR VKEILQCHSL FQIALASRVS EWDSVEMIGD VFVASFSKSM VLDAYSEYVN NFSTAVAVLK KTCATKPAFL EFLKQEQEAS PDRTTLYSLM MKPIQRFPQF ILLLQDMLKN TSKGHPDRLP LQMALTELET LAEKLNERKR DADQRCEVKQ IAKAINERYL NKLLSSGSRY LIRSDDMIET VYNDRGEIVK TKERRVFMLN DVLMCATVSS RPSHDSRVMS SQRYLLKWSV PLGHVDAIEY GSSAGTGEHS RHLAVHPPES LAVVANAKPN KVYMGPGQLY QDLQNLLHDL NVIGQITQLI GNLKGNYQNL NQSVAHDWTS GLQRLILKKE DEIRAADCCR IQLQLPGKQD KSGRPTFFTA VFNTFTPAIK ESWVNSLQMA KLALEEENHM GWFCVEDDGN HIKKEKHPLL VGHMPVMVAK QQEFKIECAA YNPEPYLNNE SQPDSFSTAH GFLWIGSCTH QMGQIAIVSF QNSTPKVIEC FNVESRILCM LYVPVEEKRR EPGAPPDPET PAVRASDVPT ICVGTEEGSI SIYKSSQGSK KVRLQHFFTP EKSTVMSLAC TSQSLYAGLV NGAVASYARA PDGSWDSEPQ KVIKLGVLPV RSLLMMEDTL WAASGGQVFI ISVETHAVEG QLEAHQEEGM VISHMAVSGV GIWIAFTSGS TLRLFHTETL KHLQDINIAT PVHNMLPGHQ RLSVTSLLVC HGLLMVGTSL GVLVALPVPR LQGIPKVTGR GMVSYHAHNS PVKFIVLATA LHEKDKDKSR DSLAPGPEPQ DEDQKDALPS GGAGSSLSQG DPDAAIWLGD SLGSMTQKSD LSSSSGSLSL SHGSSSLEHR SEDSTIYDLL KDPVSLRSKA RRAKKAKASS ALVVCGGQGH RRVHRKARQP HQEELAPTVM VWQIPLLNI
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARHGEF10 (rho Guanine Nucleotide Exchange Factor (GEF) 10 (ARHGEF10))

Alternative Name: ARHGEF10

Background: Rho guanine nucleotide exchange factor 10,FUNCTION: May play a role in developmental myelination of peripheral nerves. {ECO:0000269|PubMed:14508709}.

Molecular Weight: 151.6 kDa

UniProt: O15013