FAM135B Protein (AA 1-1406) (Strep Tag)
Crystallography grade
FAM135B
Origin: Human
Host: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
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- Target See all FAM135B products
- FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1406
- Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This FAM135B protein is labelled with Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequence
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MSEIQGTVEF SVELHKFYNV DLFQRGYYQI RVTLKVSSRI PHRLSASIAG QTESSSLHSA CVHDSTVHSR VFQILYRNEE VPINDAVVFR VHLLLGGERM EDALSEVDFQ LKVDLHFTDS EQQLRDVAGA PMVSSRTLGL HFHPRNGLHH QVPVMFDYFH LSVISVTVHA ALVALQQPLI SFTRPGRGSW LGKGGPDTGQ EQSIISLENL VFGAGYCKPT SSEGSFYITS ENCMQHAHKW HRDLCLLLLH AYRGLRLHFL VIMRDIPELP HTELEALAVE ETLSQLCSEL QMLNNPEKIA EQISKDLAWL TSHMMTLWTQ FLDTVTLHSQ VTTYLTQEHH TLRVRRFSEA FFYMEHQKLA VLTFQENLIQ THSQLSLDIR NSEYLTSMPP LPAECLDIDG DWNTLPVIFE DRYVDCPATG HNLSVYPNFD VPVTSPTIMN LKDKEDNCMV NSNLSFREDL VLSTIKPSQM DSDEEVIRCP EPGENVATQN HMDMCSESQV YISIGEFQNK AGVPEDECWT GQTSDAGTYP VADVDTSRRS PGPEDGQAPV LTYIDVKSSN KNPSRAEPLV AFNAQHESRS SRDKYGLDRT GLSKVVVGGS HQNAISSDKT TLHELSTLGK GIDQEGKMVL LSLKLTPSEP CDPLSSTLRE PLDIRSSLKD SHTEEQEELS VLSGVIKRSS SIISDSGIES EPSSVAWSEA RSRALELPSD REVLHPFVRR HALHRNSLEG GHTESNTSLP SGIQASLTSI SSLPFEEDER EVALTKLTKS VSAPHISSPE EAAEDADTKQ QDGGFAEPSD MHSKSQGSPG SCSQLCGDSG TDAGADHPLV EIVLDADNQQ GPGYIDIPKG KGKQFDAQGH CLPDGRTENT PGVETKGLNL KIPRVIALEN PRTRSLHRAL EETPKGMPKD LNVGQQALSN SGISEVEGLS QHQVPELSCT SAADAINRNS TGQQSQSGSP CIMDDTAFNR GVNAFPEAKH KAGTVCPTVT HSVHSQVLKN QELKAGTSIM GSHLTSAETF TLDSLKAVEV VNLSVSCTAT CLPFSSVPKE TPARAGFSSK QTLFPITHQP LGSFGVVSTH SSTLDEEVSE RMFSFYQAKE KFKKELKIEG FLYSDLTVLA SDIPYFPPEE EEENLEDGIH LVVCVHGLDG NSADLRLVKT FIELGLPGGK LDFLMSEKNQ MDTFADFDTM TDRLLDEIIQ HIQLYNLSIS RISFIGHSLG NIIIRSVLTR PRFRYYLNKL HTFLSLSGPH LGTLYNNSTL VSTGLWLMQK LKKSGSLLQL TFRDNADLRK CFLYQLSQKT GLQYFKNVVL VASPQDRYVP FHSARIEMCK TALKDRHTGP VYAEMINNLL GPLVEAKDCT LIRHNVFHAL PNTANTLIGR AAHIAVLDSE LFLEKFFLVA GLNYFK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))
- Alternative Name
- FAM135B (FAM135B Products)
- Synonyms
- MGC81353 Protein, C8ORFK32 Protein, RGD1308133 Protein, 1700010C24Rik Protein, A830008O07Rik Protein, family with sequence similarity 135 member B Protein, family with sequence similarity 135 member B L homeolog Protein, family with sequence similarity 135, member B Protein, FAM135B Protein, fam135b.L Protein, Fam135b Protein
- Background
- Protein FAM135B
- Molecular Weight
- 155.8 kDa
- UniProt
- Q49AJ0
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