NUBP1 Protein (AA 1-320) (Strep Tag)

Catalog No. ABIN3119841

This Recombinant NUBP1 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for NUBP1 Protein (AA 1-320) (Strep Tag) (ABIN3119841)

Target: NUBP1 (Nucleotide Binding Protein 1 (NUBP1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-320

Purification tag / Conjugate: This NUBP1 protein is labelled with Strep Tag.

Sequence: MEEAPHGCPG ADSAQAGRGA SCQGCPNQRL CASGAGAAPD PAVEEIREKM KTVRHKLLVL SGKGGVGKST FSAHLAHGLA EDGDTQVALL DIDICGPSIP KIMGLEGEQV HQSGSGWSPV YVDDNLGVMS VGFLLSSPDD AVIWRGPKKN GMIKQFLRDV DWGDVDYLIV DTPPGTSDEH LSVVQYLAAA HIDGAVILTT PQEVALQDVR KEISFCHKVK LPIIGVVENM SGFICPKCKK ESQIFPPTTG GAEAMCQDLR IPLLGKVPLD PHIGKSCDKG QSFFVEAPDS PATAAYRSII QRIRDFCNSH QSHAETLISP
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NUBP1 (Nucleotide Binding Protein 1 (NUBP1))

Alternative Name: Nubp1

Background: Cytosolic Fe-S cluster assembly factor NUBP1 (Nucleotide-binding protein 1) (NBP 1),FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (By similarity). Implicated in the regulation of centrosome duplication (PubMed:16638812, PubMed:23807208). Negatively regulates cilium formation and structure (PubMed:23807208). {ECO:0000255|HAMAP-Rule:MF_03038, ECO:0000269|PubMed:16638812, ECO:0000269|PubMed:23807208}.

Molecular Weight: 34.1 kDa

UniProt: Q9R060

Pathways: Transition Metal Ion Homeostasis