FBXO5 Protein (AA 1-421) (Strep Tag)

Catalog No. ABIN3124183

This Recombinant FBXO5 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for FBXO5 Protein (AA 1-421) (Strep Tag) (ABIN3124183)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-421

Purification tag / Conjugate: This FBXO5 protein is labelled with Strep Tag.

Sequence: MSRRTCSDLR RPSSCPCRLG ARTTVDGCKE ESPVLSVTMK CFNCNPDLSE LEVVKPEDSG IEASYSPVCL EPSCNDCVRN HERLSFIDSP IVGHDNKENQ RVQNTLDSSN ETEELEASRL YEDSGYSSFT QSDRDDGILI LENFRNSPQA RLLPSQSPDQ HPNKTLLPVL HFERVVCSTL KKNGKRNPKV DREMLKEVIA SGNFRLQNII GKKMGLEHLD ILAELSRRGF VHLLANILTK LSGMDLVNLS KVSRIWKKIL ENNKGAFQLY SKTMQRVIES SKLSLHATTR GYVVGRAALT CVQKSSTWAP PKKDVQIKSS SQRGQRVSTY SRHNEFVEVA KTLKNNESLK ACVRCNFPAK YDHYLERAVC KRESCQFEYC TKCLCAYHNN KDCLNGKILK ASCKVGPLPG TKKSKKNLQR L
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: Fbxo5

Background: F-box only protein 5 (Early mitotic inhibitor 1),FUNCTION: Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:17190794, PubMed:15526037, PubMed:16809773). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:16809773). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box co-receptor, formed by FZR1 and ANAPC10, by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (By similarity). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (PubMed:15526037). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (PubMed:17190794). Promotes migration and osteogenic differentiation of mesenchymal stem cells (By similarity). {ECO:0000250|UniProtKB:Q9UKT4, ECO:0000269|PubMed:15526037, ECO:0000269|PubMed:16809773, ECO:0000269|PubMed:17190794}.

Molecular Weight: 47.5 kDa

UniProt: Q7TSG3

Pathways: Mitotic G1-G1/S Phases