NPHP4 Protein (AA 1-1425) (Strep Tag)

Catalog No. ABIN3134553

This Recombinant NPHP4 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for NPHP4 Protein (AA 1-1425) (Strep Tag) (ABIN3134553)

Target: NPHP4 (Nephronophthisis 4 (NPHP4))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1425

Purification tag / Conjugate: This NPHP4 protein is labelled with Strep Tag.

Sequence: MGDWHRAFTQ NTLVPPHPQR ARQLGKESTA FQCILKWLDG PLIKQGILDM LSELECHLRV TLFDVTYKHF FGRTWKTTVK PTNQPSKQPP RITFNEPLYF HTTLSHPSIV AVVEVVTEGR KRDGTLQLLS CGFGILRIFG NKPESPTSAA QDKRLRLYHG TPRALLHPLL QDPIEQNKFM RLMENCSLQY TLKPHPPLEP AFHLLPENLL VSGFQQIPGL LPPHGDTGDA LRKPRFQKPT TWHLDDLFFT LYPSLEKFEE ELVQLLISDR EGVGLLDSGT LEVLERRLHV CVHNGLGFVQ RPQVVVLVPE MDVALTRSAS FSRKISASSK NSSGNQALVL RSHLRLPEMV SHPAFAIVFQ LEYVFNSPSG ADGGASSPTS ISSVACMHMV RWAVWNPDLE VGPGKVTLPL QGGVQQNPSR CLVYKVPSAS MSSEEVKQVE SGTIQFQFSL SSDGPTEHAN GPRVGRRSSR KMPASPSGTP APAARDLAAT QDSPVGPGLS LSQLTASPLS PALQSSSKPP LQPPDSSQSP EGPQLQAESV LESRVSHLEA DLSQPASLQG TPAVEHLQEL PFTPLHAPIV VGAQTRSSRS QLSRAAMVLL QSSGFPEILD ASQQPVEAVN PIDPVRFNPQ KEESDCLRGN EIVLQFLAFS RAAQDCPGTP WPQTVYFTFQ FYRFPPETTP RLQLVKLDGT GKSGSGSLSH ILVPINKDGS FDAGSPGLQL RYMVDPGFLK PGEQRWFAHY LAAQTLQVDV WDGDSLLLIG SAGVQMKHLL RQGRPAVQVS HELEVVATEY EQEMMAVSGD VAGFGSVKPI GVHTVVKGRL HLTLANVGHA CEPRARGSNL LPPSRSRVIS NDGASFFSGG SLLIPGGPKR KRVVQAQRLA DVDSELAAML LTHTRAGQGP QAAGQEADAV HKRKLERMRL VRLQEAGGDS DSRRISLLAQ HSVRAQHSRD LQVIDAYRER TKAESIAGVL SQAITTHHTL YATLGTAEFF EFALKNPHNT QHTVAIEIDS PELSIILDSQ EWRYFKEATG LHTPLEEDMF HLRGSLAPQL YLRPRETAHI PLKFQSFSVG PLAPTQAPAE VITEKDAESG PLWKCSAMPT KHAKVLFRVE TGQLIAVLCL TVEPQPHVVD QVFRFYHPEL TFLKKAIRLP PWHTLPGAPV GMPGEDPPVH VRCSDPNVIC EAQNVGPGEP RDVFLKVASG PSPEIKDFFV VIYADRWLAV PVQTWQVCLH SLQRVDVSCV AGQLTRLSLV LRGTQTVRKV RAFTSHPQEL KTDPAGVFVL PPHGVQDLHV GVRPRRAGSR FVHLNLVDID YHQLVASWLV CLSCRQPLIS KAFEITMAAG DEKGTNKRIT YTNPYPSRRT YRLHSDRPEL LRFKEDSFQV AGGETYTIGL RFLPSGSAGQ EEILIYINDH EDKNEETFCV KVLYQ
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NPHP4 (Nephronophthisis 4 (NPHP4))

Alternative Name: Nphp4

Background: Nephrocystin-4 (Nephroretinin),FUNCTION: Involved in the organization of apical junctions, the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis (By similarity). Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1 (By similarity). In cooperation with INVS may down-regulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling (By similarity). Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ (By similarity). {ECO:0000250|UniProtKB:B0DOB4, ECO:0000250|UniProtKB:O75161}.

Molecular Weight: 157.3 kDa

UniProt: P59240