IFIT1 Protein (AA 1-463) (Strep Tag)

Catalog No. ABIN3135587

This Recombinant IFIT1 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for IFIT1 Protein (AA 1-463) (Strep Tag) (ABIN3135587)

Target: IFIT1 (Interferon-Induced Protein with Tetratricopeptide Repeats 1 (IFIT1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-463

Purification tag / Conjugate: This IFIT1 protein is labelled with Strep Tag.

Sequence: MGENADGDQV MENLLQLRCH FTWKLLFENN DIPDLEVRIS EQVQFLDIKN PLGMHNLLAY VRHLKGQQDE ALQSLKEAEA LIQSEQLSKR SLATWGNCAW LHYHRGSLAE AQIYLDKVEK VCKEFSSPFR YRLECAEMDC EEGWALLKCG GGNYKQAMAC FAKALKVEPE NPEYNTGYAV VAYRQDLDDN FISLEPLRKA VRLNPEDPYL KVLLALKLQD LGEHVEAEAH IEEALSSTSC QSYVIRYAAK YFRRKHRVDK ALHLLNRALQ ASPSSGYLHY QKGLCYKQQI SQLRTSRNRQ PRRQDNVQEL AQQAIHEFQE TLKLRPTFEM AYVCMAEVQA EIHQYEEAER NFQKALNNKT LVAHIEQDIH LRYGRFLQFH KQSEDKAITL YLKGLKVEEK SFAWRKLLTA LEKVAERRVC QNVHLVESTS LLGLVYKLKG QEKNALFYYE KALRLTGEMN PAF
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: IFIT1 (Interferon-Induced Protein with Tetratricopeptide Repeats 1 (IFIT1))

Alternative Name: Ifit1

Background: Interferon-induced protein with tetratricopeptide repeats 1 (IFIT-1) (Glucocorticoid-attenuated response gene 16 protein) (GARG-16) (Interferon-induced 56 kDa protein) (IFI-56K) (P56),FUNCTION: Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). {ECO:0000269|PubMed:21085181, ECO:0000269|PubMed:22589727}.

Molecular Weight: 53.7 kDa

UniProt: Q64282

Pathways: Hepatitis C