Ephrin A1 Protein (EFNA1) (AA 1-1127) (Strep Tag)

Catalog No. ABIN3136341

This Recombinant Ephrin A1 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for Ephrin A1 Protein (EFNA1) (AA 1-1127) (Strep Tag) (ABIN3136341)

Target: Ephrin A1 (EFNA1)

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1127

Purification tag / Conjugate: This Ephrin A1 protein is labelled with Strep Tag.

Sequence: MVLSGVDKMI RLQKNTANIR NICVLAHVDH GKTTLADCLI SSNGIISSRL AGKLRYMDSR EDEQVRGITM KSSAISLHYA EGHEEYLINL IDSPGHVDFS SEVSTAVRIC DGCIIVVDAV EGVCPQTQAV LRQAWLENIR PVLVINKIDR LIVELKFTPQ EAYSHLKNIL EQINALTGTL FTSKVLEERA ERETESQAKP HSEQGEQVYD WSAGLEDVDD SQLYFSPEQG NVVFTSAIDG WGFGIEHFAR IYSQKIGIKK EVLLKTLWGD YYINMKAKKI MKVDQAKGKK PLFVQLILEN IWSLYDAVLK KDKEKIDKIV TSLGLKIGAR EARHSDPKVQ INAICSQWLP ISHAVLAMVC HKLPSPLDMT SERVEKLLCT GSQTFESLPP ETQALKAAFM KCGSEDTAPV IIFVSKMFAV DVKALPQNKP RPLTQEEMAQ RRERARQRHA EKLAAAQGQT SQGPTQDGGA LETSPHEDEP RGDEPDVASV SRQPVSQEES SQEAFIAFAR VFSGIARRGK KIFVLGPKYS PVDFLQRVPL GFSAPLEDLP PVPHMACCTL ENLYLLMGRE LEDLEEVPPG NVLGIGGLQD FVLKSATLCS LPSCPPFIPL NFEATPIVRV AVEPKHPSEM PQLVKGMKLL NQADPCVQVL IQETGEHVLV TAGEVHLQRC LDDLRERFAK IHISVSEPII PFRETITKPP KVDMVNEEIG RQQKVAVIHQ TKEEQSKIPE GIHVDSDGLI TIPTPNKLAT LSVRAIPLPE EVTRILEENS DLIRSMELLT SSLNEGRNTQ AIHQKTQEKI WEFKGKLEKH LTGRKWRNTV DQIWSFGPRK CGPNILVSRS EDFQNSVWSG PAGRESKEAS RFRDFGNSIV SGFQLATLSG PMCEEPLMGV CFVLEKWELN KCAEQGASDK QHQGQCDLAG EGQGGGKTCH VGDENQEQQD VCSEPFEETS QKGDSPVIDC YGPFSGQLIA TMKEACRYAL QVKPQRLMAA MYTCDIMATS DVLGRVYAVL SKREGRVLQE EMKEGTDMFI IKAVLPVAES FGFADEIRKR TSGLASPQLV FSHWEVIPSD PFWVPTTEEE YLHFGEKADS ENQARKYMNA VRKRKGLYVE EKIVEHAEKQ RTLSKNK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Ephrin A1 (EFNA1)

Alternative Name: Efl1

Background: Elongation factor-like GTPase 1 (Elongation factor Tu GTP-binding domain-containing protein 1) (Elongation factor-like 1) (Protein FAM42A),FUNCTION: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits (By similarity). {ECO:0000250}.

Molecular Weight: 125.8 kDa

UniProt: Q8C0D5

Pathways: RTK Signaling