Naip6 Protein (AA 1-1403) (Strep Tag)

Catalog No. ABIN3137270

Recombinant Naip6 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for Naip6 Protein (AA 1-1403) (Strep Tag) (ABIN3137270)

Target: Naip6 (NAIP7) (NLR Family, Apoptosis Inhibitory Protein 7 (NAIP7))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1403

Purification tag / Conjugate: This Naip6 protein is labelled with Strep Tag.

Sequence: MAEHGESSED RISEIDYEFL AELSARFGMN LVQLAKSQEE EDHKERMKMK KGFNSQMRSE AKRLKTFESY DTFRSWTPQE MAAAGFYHTG VKLGVQCFCC SLILFGNSLR KLPIERHKKL RPECEFLQGK DVGNIGKYDI RVKSPEKMLR GGKARYHEEE ARLESFEDWP FYAHGTSPRA LSAAGFVFTG KRDTVQCFSC GGSLGNWEEG DDPWKEHAKW FPKCEFLQSK KSSEEIAQYI QDYEGFVHVT GEHFVKSWVR RELPMVSAYC NDSVFTNEEL RMDMFKDWPQ ESPVGFEALV RAGFFYTGKK DIVRCFSCGG CLEKWAEGDD PMEDHIKFFP ECVFLQTLKS SAEVIPTLQS QYALPEATET TRESNHDDAA AVHSTVVDLG RSEAQWFQEA RSLSEQLRDT YTKTSFCHMN LPEVCSSLGT DHLLGCDVSI ISKHVSQPVQ GALTIPEVFS NLSSVMCVEG EAGSGKTTFL KRIAFLWASG CCPLLYRFQL VFYLSLSSIT PDQGLANIIC TQLLGAGGCI SEVCLSSSIQ QLQHQVLFLL DDYSGLASLP QALHTLITKN YLFRTCLLIA VHTNRVRDIR PYLGTSLEIQ EFPFYNTVFV LRKFFSHDII CVEKLIIYFS ENKDLQGVYK TPLFVAAVCN DWNQNASAQD DFQDVTLFHS YMQYLSLKYK ATAESLQATV SSCGQLALTG LFSSCFEFNS DDLAEAGVDE DVKLTTFLMS KFTAQRLRPV YRFLGPLFQE FLAAVRLTEL LSSDRQEDQD LGLYYLRQID SPLKAINSFN IFLYYVSSHS SSKAAPTVVS HLLQLVDEKE SLENMSENED YMKLHPQTFL WFQFVRGLWL VSPESFSSFV SEHLLRLALI FAYESNTVAE CSPFILQFLR GRTLALRVLN LEYFWDHPES LLLLRSLKVS INGNKMSSYV DYSFKTYFEN LQPPAINEEY TSAFEHVSEW RRNFAQDEEI IKNYENIWPR ALPDISEGYW NLSPKPCKIP KLEVQVNNMG PADQALLQVL MEVFSASQSI EFHLFNSSGF LESIRPALEL SKASVTKCSM SRLELSRAEQ ELLLTLPALQ SLEVSETNQL PDQLFHNLHK FLGLKELCVR LDGKPDVLSV LPEEFLNLHH MEKLSIRTST ESDLSKLVKF IQNFPNLHVF HLKCDFLSNC ESLMTALASC KKLREIEFSG QCFEAMTFVN ILPNFVSLKI LSLKGQQFAD KETSEKFAQA LGSLRNLEEL LVPTGDGIHQ VAKLIVRQCL QLPCLRVLAF HDILDDESVI EIARAATSGS FQKLENLDIS MNHKITEEGY RNFFQALDNL PNLQMLNICR NIPGRIQVQA TTVKALGHCV SRLPSLTRLG MLSWLLDEED MKVINDVKER HPQSKRLTIF WKWIVPFSPV VLE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Naip6 (NAIP7) (NLR Family, Apoptosis Inhibitory Protein 7 (NAIP7))

Alternative Name: Naip6

Background: Baculoviral IAP repeat-containing protein 1f (Neuronal apoptosis inhibitory protein 6),FUNCTION: Sensor component of the NLRC4 inflammasome that specifically recognizes and binds flagellin from pathogenic bacteria. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (PubMed:21874021). The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri. May contribute to prevent motor-neuron apoptosis induced by a variety of signals (By similarity). {ECO:0000250|UniProtKB:Q13075, ECO:0000269|PubMed:21874021}.

Molecular Weight: 159.9 kDa

UniProt: Q9JIB6