C2CD2L Protein (AA 1-706) (Strep Tag)

Catalog No. ABIN3124403

Recombinant C2CD2L protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for C2CD2L Protein (AA 1-706) (Strep Tag) (ABIN3124403)

Target: C2CD2L (C2CD2-Like (C2CD2L))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-706

Purification tag / Conjugate: This C2CD2L protein is labelled with Strep Tag.

Sequence: MDPDWGQRDV GWAALLVLFA ASLITVLGWM LQYARGLWLS RADGGRDSRP ASAAEPGGSL RELGVWRSLL RLRATRTSTP EEAGVRGLLA SLFAFKSFRE NWQRAWVRAL NEQACRDGSS IQIAFEEIPQ LPPRASISHV TCVDQSERTM VLHCQLSAEE VRFPISVTQQ SPAAVSMETY HVTLTLPPTQ LEVSLEEIPD EGLLVSWAFT DRPELSLKVL PKLQTRERDE EQPELSTVEE LIKDAIVSTQ PAMMVNLRAC SAPGGLVPSE KPPTMSQAQP SIPRPTRLFL RQLRASHLGS ELGGTEELCC AAELDNPMQQ KWTKPMRAGP EVEWTEDLAL DLGPQSRELT LKVLRSSSCG DAELLGQATL PVGSPSRPMS RRQVCPLTPG PGKSLSPAAT VTAELHYEQG SPRNLGTPTS STPRPSITPT KKIELDRTIM PDGTVVTTVT TVQSRPRVDG KLDSPSRSPS KVEVTEKMTT VLSESSGPSN ASHSSSRESH LSNGLDPVAE TAIRQLTEPS GRAAKKTPTK RSTLIISGVS KVPIAQDELA LSLGYAASLE ASMQDDAGTS GGPSSPPSDP SATSPGPVDA LSSPTSVQEA DETTRSDISE RPSVDDVESE TGSTGALETR SLKDHKVSFL RSGTKLIFRR RPRQKEAGLS QSHDDLSNTT ATPSVRKKAG SFSRRLIKRF SFKSKPKANG NPSPQL
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: C2CD2L (C2CD2-Like (C2CD2L))

Alternative Name: C2cd2l

Background: Phospholipid transfer protein C2CD2L (C2 domain-containing protein 2-like) (Transmembrane protein 24),FUNCTION: Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane (By similarity). It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling (By similarity). Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling: localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers (By similarity). In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane (By similarity). Positively regulates insulin secretion in response to glucose (PubMed:24012759). Phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules (By similarity). {ECO:0000250|UniProtKB:O14523, ECO:0000269|PubMed:24012759}.

Molecular Weight: 76.3 kDa

UniProt: Q80X80