LRRC8B Protein (AA 1-803) (Strep Tag)

Catalog No. ABIN3135185

This Recombinant LRRC8B protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for LRRC8B Protein (AA 1-803) (Strep Tag) (ABIN3135185)

Target: LRRC8B (Leucine Rich Repeat Containing 8 Family, Member B (LRRC8B))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-803

Purification tag / Conjugate: This LRRC8B protein is labelled with Strep Tag.

Sequence: MITLTELKCL ADAQSSYHIL KPWWDVFWYY ITLIMLLVAV LAGALQLTQS RVLCCLPCKV EFDNQCAVPW DLLKGSENAS SNSGLLLPLP LRIQNDLHRQ QYSYIDAVCY EKQLHWFAKF FPYLVLLHTL IFAACSNFWL HYPSTSSRLE HFVSILHKCF DSPWTTRALS ETVAEQSVRP LKLSKSKTLL STSGGSADID ASKQSLPYPQ PGLESPGIES PTSSVLDKKE GEQAKAIFEK VKRFRLHVEQ RDIIYRVYLK QIIVKVILFV LIITYVPYFL SYITLEIDCS IDVQAFTGYK RYQCVYSLAE IFKVLASFYV ILVMLYGLTS SYSLWWMLRS SLKQYSFEAL REKSNYSDIP DVKNDFAFIL HLADQYDPLY SKRFSIFLSE VSENKLKQIN LNNEWTVERL KSKLVKNSQD KVELHLFMLN GLPDNVFELT EMEVLSLELI PEVKLPAAVA QLVNLRELHV YHSSLVVDHP ALAFLEENLR ILRLKFTEMG KIPRWVFHLK NLKELYLSGC VLPEQLSSLH LEGFQDLKNL RTLYLKSSLS RIPQVVTDLL PSLQKLSLDN EGSKLVVLNN LKKMVNLKSL ELLSCDLERI PHSIFSLNNL HELDLKENNL KTVEEIISFQ HLPSLSCLKL WHNNIAYIPA QIGALSNLEQ LFLGHNNIES LPLQLFLCTK LHYLDLSYNH LTFIPEEIQY LTNLQYFAVT NNNIEMLPDG LFQCKKLQCL LLGRNSLTDL SPLVGELSNL THLELTGNYL ETLPVELEGC QSLKRSCLIV EDSLLNSLPL PVAERLQTCL DKC
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: LRRC8B (Leucine Rich Repeat Containing 8 Family, Member B (LRRC8B))

Alternative Name: Lrrc8b

Background: Volume-regulated anion channel subunit LRRC8B (Leucine-rich repeat-containing protein 8B),FUNCTION: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E), channel characteristics depend on the precise subunit composition. {ECO:0000250|UniProtKB:Q6P9F7}.

Molecular Weight: 92.2 kDa

UniProt: Q5DU41