ABCB11 Protein (AA 1-1321) (Strep Tag)

Catalog No. ABIN3137386

Product Details

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Protein Type: Recombinant

Protein Characteristics: AA 1-1321

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This ABCB11 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Brand: AliCE®

Sequence: MSDSVILRSV KKFGEENHAF ESDGFHNNDK KSRLQDKKKG EGARVGFFEL FRFSSSKDNW LMFMGSVCAL LHGMAQPGMI IVFGILTDIF VEYDIERQEL SIPEKVCMNN TIVWINSSFN QNMTNGTSCG LVDINSEVIK FSGIYAGVGV AVLILGYFQI RLWVITGARQ IRKMRKFYFR RIMRMEIGWF DCTSVGELNS RFSDDINKID EAIADQMALF LQRLSTALSG LLLGFYRGWK LTLVILAVSP LIGIGAAVIG LSVAKFTELE LKAYAKAGSI ADEVLSSIRT VAAFGGENKE VERYEKNLMF AQRWGIWKGM VMGFFTGYMW CLIFFCYALA FWYGSRLVLD EGEYTPGTLI QIFLCVIIAA MNIGNASSCL EIFSTGCSAA SSIFQTIDRQ PVMDCMSGDG YKLDRIKGEI EFHNVTFHYP SRPEVKILNN LSMVIKPGET TAFVGSSGAG KSTALQLIQR FYDPCEGMVT LDGHDIRSLN IRWLRDQIGI VEQEPVLFST TIAENIRLGR EEATMEDIVQ AAKDANAYNF IMALPQQFDT LVGEGGGQMS GGQKQRVAIA RALIRKPKIL LLDMATSALD NESEAKVQGA LNKIQHGHTI ISVAHRLSTV RSADVIIGFE HGTAVERGTH EELLERKGVY FMLVTLQSQE DNTHKETGIK GKDTTEGDTP ERTFSRGSYQ DSLRASIRQR SKSQLSHLSH EPPLAIGDHK SSYEDRKDND VLVEEVEPAP VRRILKYNIS EWPYILVGAL CAAINGAVTP IYSLLFSQIL KTFSLVDKEQ QRSEIYSMCL FFVILGCVSL FTQFLQGYNF AKSGELLTKR LRKFGFKAML RQDIGWFDDL KNNPGVLTTR LATDASQVQG ATGSQVGMMV NSFTNIFVAV LIAFLFNWKL SLVISVFFPF LALSGAVQTK MLTGFASQDK EILEKAGQIT NEALSNIRTV AGIGVEGRFI KAFEVELEKS YKTAIRKANV YGLCYAFSQG ISFLANSAAY RYGGYLIVYE DLNFSYVFRV VSSIAMSATA VGRTFSYTPS YAKAKISAAR FFQLLDRKPP IDVYSGAGEK WDNFQGKIDF IDCKFTYPSR PDIQVLNGLS VSVDPGQTLA FVGSSGCGKS TSIQLLERFY DPDQGTVMID GHDSKKVNVQ FLRSNIGIVS QEPVLFDCSI MDNIKYGDNT KEISVERAIA AAKQAQLHDF VMSLPEKYET NVGIQGSQLS RGEKQRIAIA RAIVRDPKIL LLDEATSALD TESEKTVQLA LDKAREGRTC IVIAHRLSTI QNSDIIAVMS QGVVIEKGTH KKLMDQKGAY YKLVITGAPI S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Alternative Name: Abcb11 (ABCB11 Products)

Background: Bile salt export pump (EC 7.6.2.-) (ATP-binding cassette sub-family B member 11) (Sister of P-glycoprotein),FUNCTION: Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:14570929, PubMed:11172067, PubMed:23764895, PubMed:22619174, PubMed:19228692). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (By similarity). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (By similarity). {ECO:0000250|UniProtKB:O95342, ECO:0000269|PubMed:11172067, ECO:0000269|PubMed:14570929, ECO:0000269|PubMed:19228692, ECO:0000269|PubMed:22619174, ECO:0000269|PubMed:23764895}.

Molecular Weight: 146.7 kDa

UniProt: Q9QY30