CA12 Protein (AA 25-301) (His tag)
Quick Overview for CA12 Protein (AA 25-301) (His tag) (ABIN4371596)
Target
See all CA12 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 25-301
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Purification tag / Conjugate
- This CA12 protein is labelled with His tag.
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Purpose
- Recombinant Mouse Carbonic Anhydrase 12/CA12 (C-6His)
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Sequence
- APLNGSKWTY VGPAGEKNWS KKYPSCGGLL QSPIDLHSDI LQYDASLAPL QFQGYNVSVE KLLNLTNDGH SVRLNLNSDM YIQGLQPHHY RAEQLHLHWG NRNDPHGSEH TVSGKHFAAE LHIVHYNSDL YPDFSTASDK SEGLAVLAVL IEIGSANPSY DKIFSHLQHV KYKGQQVLIP GFNIEELLPE SPGEYYRYEG SLTTPPCYPT VLWTVFRNPV QISQEQLLAL ETALYFTHMD DPTPREMINN FRQVQKFDER LVYISFRQGL LTDTGLSVDH HHHHH
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Characteristics
- Recombinant Mouse Carbonic Anhydrase XII is produced with a mammalian expression system in human cells. The target protein is expressed with sequence (Ala25-Ser301) of Mouse Carbonic Anhydrase XII fused with a 6His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (His tag)
CA12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase 12 (CA12) (AA 25-299) protein (His tag)CA12 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase 12 (CA12) protein (His tag)CA12 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Carbonic Anhydrase 12 (CA12) (AA 1-354) protein (GST tag)CA12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (Fc Tag)CA12 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Carbonic Anhydrase 12 (CA12) (Active) protein (His tag)CA12 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Carbonic Anhydrase 12 (CA12) (AA 25-301) (Active) protein (His tag)CA12 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, EAA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl, pH 8.0.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CA12 (Carbonic Anhydrase 12 (CA12))
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Alternative Name
- Carbonic Anhydrase XII
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Molecular Weight
- 32.4 kDa
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UniProt
- Q8CI85
Target
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