Albumin Protein (ALB) (FITC)

Catalog No. ABIN457597

Recombinant Albumin protein expressed in Cow.

Quick Overview for Albumin Protein (ALB) (FITC) (ABIN457597)

Target: Albumin (ALB)

Protein Type: Recombinant

Origin: Cow

Source: Cow

Application: Staining Methods (StM)

Purity: > 90 %

Product Details

Purification tag / Conjugate: This Albumin protein is labelled with FITC.

Characteristics: Fluorescein isothiocyanate-conjugated bovine serum albumin, Cohn fraction V. Source: Pooled normal bovine serum

Purification: Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immuno-precipitating techniques, this product contains traces transferrin and some alpha-proteins.

Application Details

Application Notes: As an efficient counterstain for cell an tissue substrates with immunoconjugates with a label contrasting with the greenish-yellow fluorescence of BSA/FITC. The purpose of counterstaining is to enhance the optical contrast of the immuno-specific staining against a counterstained background.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: BSA concentration 10 mg/ml. Fluorochrome/BSA molar ratio (F/P) 1.2.

Buffer: Fluorochrome-coupled bovine serum albumin, fraction V, lyophilized from a solution in phosphate buffered saline (PBS pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to bovine total serum proteins, to bovine IgA, IgM and albumin

Pathways: Lipid Metabolism