Albumin Protein (ALB)

Catalog No. ABIN457602

This Albumin protein is expressed in Cow.

Quick Overview for Albumin Protein (ALB) (ABIN457602)

Target: Albumin (ALB)

Origin: Cow

Source: Cow

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Binding Studies (Bind), Immunoelectrophoresis (IEP), Immunodiffusion (ID), Haemagglutination (H)

Purity: > 90 %

Product Details

Characteristics: Source: Bovine serum

Purification: Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 35 mg/ml at the level of sensitivity of the applied immuno-precipitating techniques, this product contains traces of IgM, IgA, traces of complement C3c, transferrin and albumin. Chromatographically purified bovine albumin

Application Details

Application Notes: Purified bovine albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized, stable white powder.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. After opening the vial, the lyophilized content is reconstituted by adding 2 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.

Target Details

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Identity and purity is confirmed and tested by immunoelectrophoresis using antisera to total serum proteins and albumin.

Pathways: Lipid Metabolism